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KMID : 0381920000300040403
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Korean Journal of Microscopy
2000 Volume.30 No. 4 p.403 ~ p.412
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Localization of Anti-Actin-Gold Particles (10 nm) Labeled to Nuclear Actin of Urechis Sperm and Spermatids
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Shin Kil-Sang
Kim Ho-Jin
Kim Wan-Jong
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Abstract
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Urechis unicinctus spermatogenic cells, sperm and spermatids, prepared from testis are investigated to identify nuclear actin using amoeba monorlonal anti-actin as the first Ab and gold particles (10 nm) conjugated mouse IgG (immunogold) as the Ab marker. The Ag-Ab reactions analyzed the localization of nuclear actin of the spermatogenic cells and the immunogold particles incorporated mainly with nuclear matrices. A few immunogold particles are merged into the acrosomes and the other architectures of spermatogenic cells, such as mitochondrion and centrioles. It is often observed and there is a tendency in which the incorporated immunogold particles are increased in number in the nuclear matrices of sperm compared with that of spermatids The increments and decrements of the incorporated immunogold particles according to developmental stages and the spermatogenic architec-tures are interpreted and discussed in aspect of acrosomal function and of nuclear condensation of spermatids.
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KEYWORD
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Acrosomal process, Anti-actin, Anti-actin-gold, Nuclear condensation, Spermatids
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